Barley Malt alpha-Glucosidase. VI. Localization and Development during Barley Germination.
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منابع مشابه
Study on Barley and Malt Polyphenoloxidase. I. Chromatography of Barley, Steeped Barley, and Malt Polyphenoloxidase
A comparative chromatography of polyphenoloxidase isolated from barley, steeped barley, and green malt was made. It consisted ofextraction, precipitation, ion-exchange chromatography, gel filtration, and electrofocusing. Polyphenoloxidase was resolved into a high-molecularweight enzyme and a low-molecular-weight one. The proportion of the two enzymes underwent variations during malting. An incr...
متن کاملPurification, enzymatic characterization, and nucleotide sequence of a high-isoelectric-point alpha-glucosidase from barley malt.
High-isoelectric-point (pI) alpha-glucosidase was purified 7, 300-fold from an extract of barley (Hordeum vulgare) malt by ammonium sulfate fractionation, ion-exchange, and butyl-Sepharose chromatography. The enzyme had high activity toward maltose (k(cat) = 25 s(-1)), with an optimum at pH 4.5, and catalyzed the hydrolysis by a retaining mechanism, as shown by nuclear magnetic resonance. Acarb...
متن کاملAspects of the barley seed proteome during development and germination.
Analysis of the water-soluble barley seed proteome has led to the identification of proteins by MS in the major spots on two-dimensional gels covering the pI ranges 4-7 and 6-11. This provides the basis for in-depth studies of proteome changes during seed development and germination, tissue-specific proteomes, cultivar differences related to quality parameters, analysis of the genetic basis for...
متن کاملGermination of Barley Pollen
The artificial germination of plant pollens has met with varying success. As a whole, the pollen of the Gramineae has proved very difficult to germinate. While experiments with the pollen of many plants have resulted not only in ready germination but in satisfactory methods of preserving the pollen for considerable periods of time, the results with many of the Gramineae have been far from satis...
متن کاملPurification, Enzymatic Characterization, and Nucleotide Sequence of a High-Isoelectric-Point a-Glucosidase from Barley Malt
High-isoelectric-point (pI) a-glucosidase was purified 7,300-fold from an extract of barley (Hordeum vulgare) malt by ammonium sulfate fractionation, ion-exchange, and butyl-Sepharose chromatography. The enzyme had high activity toward maltose (kcat 5 25 s ), with an optimum at pH 4.5, and catalyzed the hydrolysis by a retaining mechanism, as shown by nuclear magnetic resonance. Acarbose was a ...
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ژورنال
عنوان ژورنال: Acta Chemica Scandinavica
سال: 1965
ISSN: 0904-213X
DOI: 10.3891/acta.chem.scand.19-1014